WebHowever, if you use the UCSC Genes annotation track, this information can be recovered by downloading the knownIsoforms.txt file for the appropriate genome. $ rsem-prepare-reference –no-polyA –gtf mm9.gtf –mapping knownIsoforms.txt –bowtie-path /sw/bowtie /data/mm9 /ref/mouse_125 http://info.gersteinlab.org/RSEQtools/workflow
Is there any "knownIsoforms.txt" file for the latest UCSC refseq …
WebEach line of should be of the form: gene_id transcript_id ( with the two fields separated by a tab character ) The map can be obtained from the UCSC table browser group: Genes and Gene Prediction Tracks table: knownIsoforms Without a map: If a reference genome and gtf is used, then RSEM uses the "gene_id" and "transcript_id" attributes in the GTF file. WebThe index is created using ENSEMBL genome fasta file. User needs to provide the URL for ENSEMBL genome located in the ENSEMBL ftp. rsemstarUscsIndex ( group = c ( "sudo", "docker" ), genome.folder = getwd (), uscs.urlgenome = NULL , uscs.gtf = NULL , uscs.urlknownIsoforms = NULL , uscs.urlknownToLocusLink = NULL , threads = 1 ) busy term
Fragment Store — SeqAn 1.4.2 documentation
WebIf you are using a GTF file for the "UCSC Genes" gene set from the UCSC Genome Browser, then the "knownIsoforms.txt" file (obtained from the "Downloads" section of the UCSC Genome Browser site) is of this format. If this option is off, then the mapping of isoforms to genes depends on whether the '--gtf' option is specified. ... WebUniversity of California, Santa Cruz WebIf you are using a GTF file for the "UCSC Genes" gene set from the UCSC Genome Browser, then the "knownIsoforms.txt" file (obtained from the "Downloads" section of the UCSC Genome Browser site) is of this format. If this option is off, then the mapping of isoforms to genes depends on whether the --gtf option is specified. busy terms