Web8 jan. 2024 · In this study, 88 intestinal microbiota samples that were collected at 11-time points were sequenced using the IonS5 TM XL platform to determine the characteristic … Web13 jun. 2024 · Polymerase chain reaction assays were performed with the 515F (5′-GTGCCAGCMGCCGCGGTAA-3′) and 907R (5′-CCGTCAATTCCTTTGAGTTT-3′) primer pair , which amplified variable regions 4 and 5 in the 16S rRNA gene, and 16S rRNA amplicon sequencing was performed with the IonS5 TM XL platform (Thermo Fisher …
Succession of oral microbiota community as a tool to estimate ...
Web2 S. Latif, R. Kousar and A. Fatima et al. / Data in Brief 42 (2024) 108057 Keywords: Triticum aestivum L. Zea mays ducedL. present Rhizosphere 16S rRNA IonS5 tm XL … WebHere, a high-throughput sequencing technique, IonS5 TM XL was employed to analyze tobacco leaves infected by D. segeticola at different disease severity levels, ranging from … china people\u0027s bank
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WebThe libraries generated with Ion Plus Fragment Library Kit 48 rxns for Thermofisher and quantified via Qubit and Q-PCR were sequenced by IonS5TMXL (Thermofisher). Single-end read was assigned to samples based on their unique barcode and truncated by cutting off the barcode and primer sequence. WebAfter the Ion S5™ or Ion S5™ XL System (Cat. nos. A27211 or A27213 ) is uncrated, installation and testing of the Ion S5™ Sequencer or Ion S5™ XL Sequencer and Torrent Server (required for use with the Ion S5™ XL Sequencer) takes ~8 hrs. The Ion Chef™ Instrument or Ion OneTouch™ 2 Instrument plus Ion OneTouch™ ES Web3 feb. 2024 · Based on the IonS5TMXL sequencing platform, the raw data was sheared and filtered, chimera was removed, and quality control was carried out to obtain the effective data (clean data) for subsequent analysis[4-5]. Operational taxonomic units (OTUs) clustering analysis was carried out by using uparse software according to 97% similarity … china people\u0027s liberation army equipment