Ions5 tm xl

Web8 jan. 2024 · In this study, 88 intestinal microbiota samples that were collected at 11-time points were sequenced using the IonS5 TM XL platform to determine the characteristic … Web13 jun. 2024 · Polymerase chain reaction assays were performed with the 515F (5′-GTGCCAGCMGCCGCGGTAA-3′) and 907R (5′-CCGTCAATTCCTTTGAGTTT-3′) primer pair , which amplified variable regions 4 and 5 in the 16S rRNA gene, and 16S rRNA amplicon sequencing was performed with the IonS5 TM XL platform (Thermo Fisher …

Succession of oral microbiota community as a tool to estimate ...

Web2 S. Latif, R. Kousar and A. Fatima et al. / Data in Brief 42 (2024) 108057 Keywords: Triticum aestivum L. Zea mays ducedL. present Rhizosphere 16S rRNA IonS5 tm XL … WebHere, a high-throughput sequencing technique, IonS5 TM XL was employed to analyze tobacco leaves infected by D. segeticola at different disease severity levels, ranging from … china people\u0027s bank https://estatesmedcenter.com

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WebThe libraries generated with Ion Plus Fragment Library Kit 48 rxns for Thermofisher and quantified via Qubit and Q-PCR were sequenced by IonS5TMXL (Thermofisher). Single-end read was assigned to samples based on their unique barcode and truncated by cutting off the barcode and primer sequence. WebAfter the Ion S5™ or Ion S5™ XL System (Cat. nos. A27211 or A27213 ) is uncrated, installation and testing of the Ion S5™ Sequencer or Ion S5™ XL Sequencer and Torrent Server (required for use with the Ion S5™ XL Sequencer) takes ~8 hrs. The Ion Chef™ Instrument or Ion OneTouch™ 2 Instrument plus Ion OneTouch™ ES Web3 feb. 2024 · Based on the IonS5TMXL sequencing platform, the raw data was sheared and filtered, chimera was removed, and quality control was carried out to obtain the effective data (clean data) for subsequent analysis[4-5]. Operational taxonomic units (OTUs) clustering analysis was carried out by using uparse software according to 97% similarity … china people\u0027s liberation army equipment

Long-term probiotic intervention mitigates memory dysfunction …

Category:Succession of oral microbiota community as a tool to estimate ...

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Ions5 tm xl

Characterization of bacterial community structure in the …

Web10 mrt. 2024 · The sequencing library was constructed using the Ion Plus Fragment Library Kit 48 rxns (Thermo Scientific). After the constructed library was qualified by Qubit … http://biotech.aiijournal.com/EN/abstract/abstract12816.shtml

Ions5 tm xl

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Web17 feb. 2024 · Cognitive impairment is associated with aging; however, the underlying mechanism remains unclear. Our previous study found that polyphenol-rich blueberry–mulberry extract (BME) had an antioxidant capability and effectively alleviated cognitive impairment in a mouse model of Alzheimer’s disease. Thus, … WebTM XL platform (Thermo Scientific, Massachusetts, USA) and 400 bp/600 bp single-end reads were generated. Sequencing data processing Quality filtering on the raw reads …

WebThe sequencing and bioinformatics showed that the analysis on IonS5TMXL sequencing platform (Thermofisher) generated 415 bp DNA fragment, which was completed by … Web7 apr. 2024 · The sequencing of samples was performed on an IonS5 TM XL sequencer by Novogene, Beijing, China. Processing of sequencing data was performed on extracted …

Web10 sep. 2024 · The sequencing platform was IonS5 TM XL. The single-end method was used to construct a small fragment library for single-terminal sequencing. 16s rRNA sequencing data processing. Web14 okt. 2024 · Here, a high-throughput sequencing technique, IonS5 TM XL was employed to analyze tobacco leaves infected by D. segeticola at different disease severity levels, ranging from T1G (least disease index) to T4G (highest disease index), in an attempt to explore the composition and diversity of phyllospheric microbiota.

Web27 okt. 2024 · The purified amplicon were constructed for single-end sequencing based on the IonS5 TM XL sequencing platform. The reads were assigned to operational taxonomic units (OTUs) with a 97% similarity threshold and taxonomy assignment of the resulting OTU was carried out using the BLAST against the UNITE reference database.

http://www.veterinaryworld.org/Vol.13/September-2024/31.html grambling state university refund scheduleWeb15 jun. 2024 · amplicons were sequenced on the IonS5TMXL platform (Thermofisher, Waltham, MA, USA). Raw reads were submitted to the National Center for Biotechnology … grambling state university race carWeb10 sep. 2024 · Shanghai Key Laboratory of Crime Scene Evidence, Shanghai Research Institute of Criminal Science and Technology, Zhongshan North No 1 Road, Shanghai, 200083, China. Scientific Reports , 10 Sep 2024, 9 (1): 13063. DOI: 10.1038/s41598-019-49338-z PMID: 31506511 PMCID: PMC6737051. Free to read & use. grambling state university radio stationWeb10 mrt. 2024 · Based on the IonS5 TM XL sequencing platform, a single-end sequencing method was used to construct a small fragment library for single-end sequencing. grambling state university quarterbacksWeb10 sep. 2024 · The sequencing platform was IonS5 TM XL. The single-end method was used to construct a small fragment library for single-terminal sequencing. 16s rRNA … grambling state university residential lifeWebQuick Order. Order Status and Tracking. Documents and Certificates. Search All. Order Status. Quick Order. Sign in. Error: This product (Catalog number A27212) has been … china people\u0027s congressWeb14 jan. 2024 · The libraries were generated using an Ion Plus Fragment Library Kit (Thermo Fisher Scientific) and quantified using Qubit and qPCR, then sequenced on an IonS5 TM XL instrument (Thermo Fisher Scientific). Single-end reads were generated. Analysis and annotation of operational taxonomic units (OTUs) grambling state university public or private